High Density Nickel

Nickel chelate resins recognize two exposed histidines for an appreciable retention of a protein and it is recommended in the majority of the cases. This resin works well with relatively easy to separate His-tagged proteins.
High Density Nickel provides a higher amount of available groups and usually allows for higher binding capacity. The resin is adequate to work in batch or column purifications (Low Flow).

REFERENCES

  • Lechner, Horst et al. “Library of Norcoclaurine Synthases and Their Immobilization for Biocatalytic Transformations.” Biotechnology Journal 13.3 (2018): n. pag. Biotechnology Journal. 
  • Marsden, A. P. et al. Investigating the Effect of Chain Connectivity on the Folding of a Beta-Sheet Protein On and Off the Ribosome. Journal of Molecular Biology 430, 5207–5216 (2018).
  • Sanchez-Rodriguez, Ana et al. “Recombinant Rabbit Beta Nerve Growth Factor Production and Its Biological Effects on Sperm and Ovulation in Rabbits.” PLoS ONE 14.7 (2019): n. pag. PLoS ONE. 
  • Product Name High Density Nickel
  • Cat. No. 6BCL-QHNi-X
  • Bead Geometry & Size Spherical Standard
  • Crosslinked Yes
  • Agarose % 6 %
  • Antimicrobial Agent 20% Ethanol
  • Storage Temperature 2-8ºC
  • Matrix Stable in all commonly used reagents
  • Binding/Loading Capacity (μmol Me2+/ml gel) 20-40
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