High Density Glyoxal 6BCL

High Density Glyoxal 6BCL allows a covalent binding of agarose to lysine amino groups. This product is adequate to work in batch or column purifications (Low Pressure). The resin has a high amount of aldehydes groups (40-60 µmoles Glyoxal /ml gel) providing a very good option to conjugate affinity ligands improving the stability of the ligand. The backbone of the resin is 6% agarose beads and the pore size is most commonly used to work with enzymes & proteins.

REFERENCES:

  • Guisan, Jose M. et al. “One-Point Covalent Immobilization of Enzymes on Glyoxyl Agarose with Minimal Physico-Chemical Modification: Immobilized ‘Native Enzymes.’” Methods in Molecular Biology. Vol. 2100. Humana Press Inc., 2020. 83–92. Methods in Molecular Biology. 
  • Product Name High Density Glyoxal 6BCL
  • Cat. No. 6BCL-GM3-X
  • Bead Geometry & Size Spherical
  • Crosslinked Yes
  • Agarose % 6 %
  • Antimicrobial Agent 20% Ethanol
  • Storage Temperature 2-8˚C
  • Standard ~50-150 µm
  • Matrix Active Groups Agarose with some diols oxidized to aldehydes
  • Activation degree (μmol glyoxyl/ml gel) 40-60
  • Coupling Capacity (mg bsa/ml gel) 20-25 (Orientative values for coupling capacity using BSA)
  • Content not available