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New treatments to cure these antibiotic-resistant bacteria are being built using customized bacteriophages that can deliver genetic material to remove selected bacterial species without affecting the overall microbiome composition.
Listeriosis is a serious gastrointestinal infection usually caused by eating food contaminated with Listeria monocytogenes which is a Gram-positive bacterium that causes sepsis and meningoencephalitis in immunocompromised adults and life-threatening maternal/fetal infections during pregnancy. An estimated 1,600 people get listeriosis each year, and about 260 die.
New treatments to cure these antibiotic-resistant bacteria are being built using customized bacteriophages that can deliver genetic material to remove selected bacterial species without affecting the overall microbiome composition. To develop effective phage therapeutics, a team of scientists at the University of Zurich has identified the highly specific receptor-binding proteins (RBPs) from Listeria monocytogenes phage PSA (Gp15) and constructed a synthetic phage library.
"ABT´S AFFINITY CHROMATOGRAPHY IS A WELL-ESTABLISHED PLATFORM USED IN RESEARCH, DEVELOPMENT, AND MANUFACTURING BIOPROCESSES "
Using Agarose Bead Technologies’ (ABT) low-density nickel immobilized metal affinity chromatography (IMAC) they were able to obtain RBP proteins for their fluorescence cell binding assays and protein crystallization.
The scientists were able to solve the crystal structure of Gp15 that help to create a blueprint for the structure-guided design of the phages with chimeric RBPs.
ABT´s affinity chromatography is a well-established platform used in research, development, and manufacturing bioprocesses to separate the molecule of interest from the crude, and it has the potential to decrease time-to-market, increase R&D productivity, and reduce cost.
In summary, the constructed library of bacteriophages can be used to find the specific RBPs for the treatment of bacterial infections. At Agarose Bead Technologies, we are proud to be contributing to the research and development of new treatments for antibiotic-resistant bacteria using our IMAC technology.
References
1. Matthew Dunne, Beatrice Rupf, Marc Tala, Xhem Qabrati, Patrick Ernst, Yang Shen, Eric Sumrall, Laura Heeb, Andreas Plückthun, Martin J. Loessner, and Samuel Kilcher. Reprogramming Bacteriophage Host Range through Structure-Guided Design of Chimeric Receptor Binding Proteins. Cell Reports, Volume 29, Issue 5. 2019. Pages 1336-1350.e4 - Link
2. https://www.sciencedirect.com/science/article/pii/S2211124719312598
3. PHOTO: Link