Sepadextran™-25 Medium

Sepadextran™ is a beaded gel filtration medium prepared by crosslinking dextran and it is supplied in its dry form. Sepadextran™-25 can be used for protein and nucleic acid purifications being the exclusion limit 5 kD for proteins and 10 bases for nucleic acids. Desalting (before IEX and after HIC or Affinity Chromatography) & buffer exchange (between different chromatography steps) are other uses.

Medium grade is suitable for separations at high flow rates and low operating pressures.
  • Product Name Sepadextran™-25 Medium
  • Cat. No. SD25M-X
  • Storage Temperature Ambient
  • Autoclavable 121˚C
  • pH Stability 2.0 to 13
  • Matrix Crosslinked Dextran
  • Dry Particle Size 50-150 µm (>80%)
  • Maximum Operating Pressure Generally obeys Darcy’s Law
  • Swelling 4-6 ml/g
  • Water Regain 2.15-2.25 ml/g
  • Wet Particle Size 85-260 µm
  • pH 7.0 (30 minutes) 1
  • including 0.1 M NaOH; 0.01 M HCl; 1 M acetic acid; 8 M urea; 6 M guanidine HCl; 1% SDS
  • 24% Ethanol; 30% Propanol; 30% Acetonitrile 1
  • Fractionation Range 1-5 kD for globular proteins
  • 000 Da for dextrans 1
  • Content not available