This study investigated the impact of Agarose Bead Technologies´ IMAC resin properties on DNA aptamer selection.
Aptamers have gained recognition for their ability to bind to a wide range of targets with high affinity and specificity which makes them an exciting tool for scientific research, clinical diagnostics, and potential therapeutic applications. These short DNA or RNA single-stranded molecules with unique structures, and offer several advantages over traditional antibodies, including lower immunogenicity, molecular weight, and synthesis cost.
To select aptamers, the systematic evolution of ligands by the exponential enrichment (SELEX, Figure 1) method is commonly employed. SELEX involves several stages, with the first step being the exposure of a single-stranded DNA aptamer library to the target molecule. Following the binding, unbound oligonucleotides are washed away, and the DNA fragments bound to the target are amplified through PCR. This process is repeated for multiple selection rounds to enrich the aptamer pool with sequences exhibiting high specificity and affinity for the target.
"THE CHOICE OF IMAC RESIN MATERIAL AND THE METAL IONS USED PLAYED A VITAL ROLE IN APTAMER SELECTION."
The success of the SELEX method relies heavily on target immobilization. Among these, immobilized metal affinity chromatography (IMAC) resin-based SELEX has emerged as one of the most popular for selecting DNA aptamers by binding the target molecules through short histidine tags, providing a simple yet efficient immobilization procedure.
This study investigated the impact of Agarose Bead Technologies´ IMAC resin properties on DNA aptamer selection. The researchers observed that the choice of IMAC resin material and the metal ions used (such as Co2+, Cu2+, Ni2+, and Zn2+) played a vital role in aptamer selection.
The results demonstrated that aptamer pools selected on copper-containing beads initially showed comparable binding to the DNA library but displayed a remarkable increase in binding affinity after subsequent SELEX rounds (figure 2).
As the field of aptamer research continues to evolve, further exploration of immobilization techniques and resin properties will undoubtedly contribute to the development of novel aptamers with exceptional specificity and affinity for diverse targets. The combination of agarose resin and aptamers holds great promise for advancing scientific understanding, diagnostics, and potentially revolutionizing modern therapeutics.