The preactivated Glyoxal Agarose base beads offered by Agarose Bead Technologies possess all the required properties of preferred supports for the preparation of affinity resins
"THIS CHEMISTRY ENABLES STABLE CONJUGATION OF LIGANDS VIA MULTIPOINT COVALENT ATTACHMENT"
The high activation level is one of the main advantages of GA support as compared to other types of activated beads. In reductive amination, ligand immobilization is driven by the higher density of reactive groups (primary amino groups, lysins) on the protein surface.
Consequently, ligands are “self-directed” onto the agarose surface by the area where multipoint covalent immobilization is more favorable.
For this reason, GA has been successfully used for the stabilization of enzymes and proteins via multipoint covalent attachment with a low impact on the properties of immobilized ligand. For instance, between 1 to 4-logs improvement in the stability of the immobilized enzymes with at least 70% of their activity have been reported for enzymes immobilized on glyoxal agarose (1).
The preactivated Glyoxal Agarose base beads possess all the required properties of preferred supports for the preparation of affinity resins. The superiority of these beads is associated with the character of glyoxal conjugation chemistry and with the vast selection of particle and pore sizes enabling either high capacities of the target and/or process scenarios.
1. Hussain, Fouzia et al., Molecules 23.12 (2018); Guisan, Jose M. et al., Methods in Molecular Biology. Vol. 2100, (2020), p. 83–92.