Low Density Nickel

Nickel chelate resins recognize two exposed histidines for an appreciable retention of a protein and it is recommended in the majority of the cases.This resin works well with relatively easy to separate His-tagged proteins.
Low Density Nickel has a lower amount of available groups that usually improve the selectivity/specificity.

REFERENCES:

  • Dunne, Matthew et al. “Reprogramming Bacteriophage Host Range through Structure-Guided Design of Chimeric Receptor Binding Proteins.” Cell Reports 29.5 (2019): 1336-1350.e4. Cell Reports. 

  • Product Name Low Density Nickel
  • Cat. No. 6BCL-QLNi-X
  • Bead Geometry & Size Spherical
  • Crosslinked Yes
  • Agarose % 6 %
  • Antimicrobial Agent 20% Ethanol
  • Storage Temperature 2-8˚C
  • Standard ~ 50-150 µm
  • Matrix Stable in all commonly used reagents
  • Binding/Loading Capacity (μmol Me2+/ml gel) 5-19
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