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Affinity Chromatography
Low Density Nickel
Nickel chelate resins recognize two exposed histidines for an appreciable retention of a protein and it is recommended in the majority of the cases.This resin works well with relatively easy to separate His-tagged proteins.
Low Density Nickel has a lower amount of available groups that usually improve the selectivity/specificity.
REFERENCES:
- Dunne, Matthew et al. “Reprogramming Bacteriophage Host Range through Structure-Guided Design of Chimeric Receptor Binding Proteins.” Cell Reports 29.5 (2019): 1336-1350.e4. Cell Reports.