Affinity Chromatography

Affinity Chromatography is a technique that separates tagged proteins and other biomolecules using biological interactions. This technique has high selectivity and is widely used to obtain proteins with high purity at high yields. ABT manufactures a variety of activated beads used for His-tag, Antibody, GST and Avidin/Streptavidin Purifications.

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Antibody Purification
Biotin/Avidin Binding Purification
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Affinity Chromatography

High Density Metal Free

6BCL-QH

This resin provides a high amount of activation for metal attachment allowing for higher binding capacity. The resin is adequate to work in batch or column purifications (Low Flow). It is supplied without any metal and it is necessary to charge the resin using the selected divalent cation following instructions. ABT offers 25ml and 100ml. For Bulk...

Affinity Chromatography

Nickel NTA Rapid Run™

This resin consists of crosslinked agarose derivatized with Nitrilotriacetic acid (NTA) and provides good properties working in native or denaturing conditions. This resin can recover His-tagged proteins from a variety of expression systems such as baculovirus, yeast, mammalian and bacterial cells. Nickel NTA Rapid Run™ provides the advantages of the...

Affinity Chromatography

Nickel Agarose Extrachel™

6RREXCH

Nickel Agarose Extrachel™ is a high capacity resin manufactured with a polychelator ligand. The product is developed to work in the presence of EDTA and DTT without any loss of performance. Its specificity and stability allows a one-step purification eliminating the need of pretratment of samples that cause nickel stripping.

Affinity Chromatography

High Density Nickel

Nickel chelate resins recognize two exposed histidine for an appreciable retention of a protein and it is recommended in the majority of the cases- this resin works well with relatively easy to separate His-tagged proteins. HIGH Density NICKEL provides a higher amount of available groups and usually allows for higher binding capacity. The resin is...

Affinity Chromatography

High Density Zinc

Zinc chelates seems to recognize two exposed histidine in vicinal position and it is recommended working with proteins that are difficult to separate. HIGH Density Zinc resin provides a high amount of available groups allowing for higher binding capacity. The resin is adequate to work in batch or column purifications (Low Flow). ABT offers 25ml and...

Affinity Chromatography

High Density Cobalt

Cobalt chelates recognize two exposed vicinal histidine tags - this resin is used in many purifications because this cation provides very good selectivity. HIGH Density COBALT provides a high amount of available groups allowing for higher binding capacity. The resin is adequate to work in batch or column purifications (Low Flow). ABT offers 25ml and...

Affinity Chromatography

Low Density Metal Free

6BCL-QL

This resin provides a low amount of activation for metal attachment allowing for higher selectivity/specificity in the binding of the tagged-protein. The resin is adequate to work in batch or column purifications (Low Flow). It is supplied without any metal and it is necessary to charge the resin using a selected divalent cation following instructions....

Affinity Chromatography

Low Density Nickel

Nickel chelate resins recognize two exposed histidine tags for an appreciable retention of a protein and it is recommended in the majority of the cases- this resin works well with relatively easy to separate His-tagged proteins. Low Density Nickel has a lower amount of available groups that usually improve the selectivity/specificity. ABT offers 25ml,...

Affinity Chromatography

Low Density Zinc

Zinc chelates seem to recognize two exposed histidine tags in vicinal position – this resin is recommended working with proteins that are difficult to separate. Low Density Zinc has a lower amount of available groups that usually improve the selectivity/specificity. The resin is adequate to work in batch or column purifications (Low Flow). ABT offers...

Affinity Chromatography

Low Density Copper

Copper chelates recognize one single exposed histidine tag – this resin is recommended working with proteins that are difficult to separate. LOW Density COPPER has a lower amount of available groups that usually improve the selectivity/specificity. The resin is adequate to work in batch or column purifications (Low Flow). ABT offers 25ml and 100ml. For...

Affinity Chromatography

His-Column High Density Nickel

Nickel chelate resins recognize two exposed histidine tags for an appreciable retention of a protein and it is recommended in the majority of the cases- this resin works well with relatively easy to separate His-tagged proteins. His-COLUMN HIGH Density NICKEL is a Pre-Packed ready to use product for gravity flow purifications. It includes 8 Pre-packed...

Affinity Chromatography

HisXL Column High Density Nickel

Nickel chelate resins recognize two exposed histidine tags for an appreciable retention of a protein and it is recommended in the majority of the cases- this resin works well with relatively easy to separate His-tagged proteins. His-XL COLUMN HIGH Density NICKEL is a Pre-Packed ready to use product for gravity flow purifications. It includes 5 Pre-packed...

Affinity Chromatography

His-Column High Density Cobalt

Cobalt chelates recognize two exposed vicinal histidine tags - this resin is used in many purifications because this cation provides very good selectivity. His-COLUMN HIGH Density COBALT is a Pre-Packed ready to use product for gravity flow purifications. It includes 8 Pre-Packed columns filled with 1ml of HIGH Density COBALT. This resin provides a higher...

Affinity Chromatography

HisXL Column High Density Cobalt

Cobalt chelates recognize two exposed vicinal histidine tags - this resin is used in many purifications because this cation provides very good selectivity. His-XL COLUMN HIGH Density COBALT is a Pre-Packed ready to use product for gravity flow purifications. It includes 5 Pre-Packed columns filled with 5ml of HIGH Density COBALT. This resin provides a...

Affinity Chromatography

Nickel NTA Agarose Resin

This resin consists of crosslinked agarose derivatized with Nitrilotriacetic acid (NTA) and provides good properties working in native or denaturing conditions. This resin can recover His-tagged proteins from a variety of expression systems such as baculovirus, yeast, mammalian and bacterial cells. ABT offers 25ml, 100ml & 500ml. Bulk quantities...

Affinity Chromatography

Metal Free Rapid Run™

6RR-QH

Metal Free Rapid Run™ provides is specially designed for large scale His-tagged protein purifications. It is supplied without any metal and it is necessary to charge the resin using the selected divalent cation following instructions.. ABT offers 25ml, 100ml, 500ml & 1000 ml. Bulk quantities available on request.

Affinity Chromatography

Cobalt Rapid Run™

6CoRR

Cobalt chelates recognize two exposed vicinal histidine tags - this resin is used in many purifications because this cation provides very good selectivity. COBALT RAPID RUN™ provides the advantages of the Cobalt cation with the high flow rates of the Rapid Run™ beads. It is specially designed for large scale His-tagged protein purifications. ABT offers...

Affinity Chromatography

Nickel Rapid Run™

6NiRR

Nickel chelate resins recognize two exposed histidine tags for an appreciable retention of a protein and it is recommended in the majority of the cases- this resin works well with relatively easy to separate His-tagged proteins. NICKEL RAPID RUN™ provides the advantages of the Nickel cation with the high flow rates of the Rapid Run™ beads. It is specially...

Nickel NTA: Low Pressure

Nickel NTA Magnetic Agarose Resin (5%)

Nickel NTA Magnetic Agarose Beads (5%) are products that allow rapid and easy small scale purification of histidine-tagged proteins. This resin consists of magnetic agarose derivatized with Nitrilotriacetic (NTA) and provides good properties working in native or denaturing conditions. The magnetic agarose beads provide a convenient, easy and quick method...

Affinity Chromatography

Biotin Agarose Resin

4BCL-BI

Biotin Agarose Resin is used for purification or removal of avidin or streptavidin samples. Biotin is immobilized through a spacer arm by means of covalent binding that minimize leakage. The binding is very strong, making it suitable for non-reversible binding applications (e.g. removal of avidin components from a sample).

Affinity Chromatography

Protein A/G Agarose Resin 4 Rapid Run™

4RRPAG

PROTEIN A/G Agarose Resin 4 RAPID RUN™ consists of a mixture of resins of Protein A and Protein G covalently bound to highly crosslinked agarose beads. It provides a very stable bond that can greatly minimize leakage of the proteins allowing for reuse of the affinity resin in several purification steps. The resin works well in High Flow conditions. ABT...

Affinity Chromatography

Protein A Agarose Resin 4 Rapid Run™

4RRPA

PROTEIN A Agarose Resin 4 RAPID RUN™ consists of rProtein A covalently bound to highly crosslinked agarose beads. rProtein A has high affinity regions that are specially bonded to the Fc region of the immunoglobulins (especially IgGs of different species). Protein A agarose beads have been widely used to purify a wide range of immunoglobulins of different...

Affinity Chromatography

Protein L Agarose Resin

4BCLPL

Protein L is an immunoglobulin-binding protein that was isolated from the bacteria Peptostreptococcus magnus and provides a convenient way to separate immunoglobulins from a variety of sources. rProtein L contains four immunoglobulin binding domains of the native protein and may be used for the purification of IgG, IgM, IgA and IgD containing kappa light...

Affinity Chromatography

Protein G Agarose Resin 4 Rapid Run™

4RRPG

PROTEIN G Agarose Resin 4 RAPID RUN™ consists of Protein G covalently bound to highly crosslinked agarose beads. It provides a very stable bond that can greatly minimize leakage of the Protein G allowing for reuse of the affinity resin in several purification steps. The resin works well in High Flow conditions. ABT offers 1ml, 5ml & 25ml. Bulk...

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