His-Tag Purification

Immobilized Metal Affinity Chromatography (IMAC) is the most widely used purification technique. It is based on the residues (i.e., histidines, cysteines, and to a lesser extent tryptophans), with transition metal cations, forming chelates. The transition metal/protein complex is then bound to chelating groups attached to the agarose resin. Elution is usually by lowering pH or performed by adding amidazole.

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Chelates: High Pressure RR™
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Affinity Chromatography

High Density Metal Free

6BCL-QH

This resin provides a high amount of activation for metal attachment allowing for higher binding capacity. The resin is adequate to work in batch or column purifications (Low Flow). It is supplied without any metal and it is necessary to charge the resin using the selected divalent cation following instructions. ABT offers 25ml and 100ml. For Bulk...

Affinity Chromatography

Nickel NTA Rapid Run™

6RR-NTANI

This resin consists of crosslinked agarose derivatized with Nitrilotriacetic acid (NTA) and provides good properties working in native or denaturing conditions. This resin can recover His-tagged proteins from a variety of expression systems such as baculovirus, yeast, mammalian and bacterial cells. Nickel NTA Rapid Run™ provides the advantages of the...

Affinity Chromatography

Nickel Agarose Extrachel™

6RREXCH

Nickel Agarose Extrachel™ is a high capacity resin manufactured with a polychelator ligand. The product is developed to work in the presence of EDTA and DTT without any loss of performance. Its specificity and stability allows a one-step purification eliminating the need of pretratment of samples that cause nickel stripping.

Affinity Chromatography

High Density Nickel

6BCL-QHNi

Nickel chelate resins recognize two exposed histidine for an appreciable retention of a protein and it is recommended in the majority of the cases- this resin works well with relatively easy to separate His-tagged proteins. HIGH Density NICKEL provides a higher amount of available groups and usually allows for higher binding capacity. The resin is...

Affinity Chromatography

High Density Cobalt

6BCL-QHCo

Cobalt chelates recognize two exposed vicinal histidine tags - this resin is used in many purifications because this cation provides very good selectivity. HIGH Density COBALT provides a high amount of available groups allowing for higher binding capacity. The resin is adequate to work in batch or column purifications (Low Flow). ABT offers 25ml and...

Affinity Chromatography

Nickel NTA Agarose Resin

6BCL-NTANi

Affinity Chromatography

Metal Free Rapid Run™

6RR-QH

Metal Free Rapid Run™ provides is specially designed for large scale His-tagged protein purifications. It is supplied without any metal and it is necessary to charge the resin using the selected divalent cation following instructions.. ABT offers 25ml, 100ml, 500ml & 1000 ml. Bulk quantities available on request.

Affinity Chromatography

Nickel Rapid Run™

6NiRR

Nickel chelate resins recognize two exposed histidine tags for an appreciable retention of a protein and it is recommended in the majority of the cases- this resin works well with relatively easy to separate His-tagged proteins. NICKEL RAPID RUN™ provides the advantages of the Nickel cation with the high flow rates of the Rapid Run™ beads. It is specially...

Affinity Chromatography

Cobalt Rapid Run™

6CoRR

Cobalt chelates recognize two exposed vicinal histidine tags - this resin is used in many purifications because this cation provides very good selectivity. COBALT RAPID RUN™ provides the advantages of the Cobalt cation with the high flow rates of the Rapid Run™ beads. It is specially designed for large scale His-tagged protein purifications. ABT offers...

Nickel NTA: Low Pressure

Nickel NTA Magnetic Agarose Resin (5%)

MAGNTANi5

Nickel NTA Magnetic Agarose Beads (5%) are products that allow rapid and easy small scale purification of histidine-tagged proteins. This resin consists of magnetic agarose derivatized with Nitrilotriacetic (NTA) and provides good properties working in native or denaturing conditions. The magnetic agarose beads provide a convenient, easy and quick method...

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His-Tag Purification

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