His-Column High Density Nickel

Product info

Cat. No.: 6BCL-QHNi-C8
Bead Geometry & Size: Spherical ~50 - 150 µm
Crosslinked: Yes
Agarose %: 6 %
Antimicrobial Agent: 20% Ethanol
Loading Capacity (μmol me2+/ml gel): 20-40
Storage Temperature: 2-8˚C
Product Name: His-Column High Density Nickel
Matrix: Stable in all commonly used reagents
Color

Reference: 6BCL-QHNi-C8

Overview

Resume

His-COLUMN HIGH Density NICKEL is a Pre-Packed ready to use product for gravity flow purifications. It includes 8 Pre-packed columns filled with 1ml of HIGH Density NICKEL. This resin provides a higher amount of available groups and usually allows for higher binding capacity.

Description

Nickel chelate resins recognize two exposed histidines for an appreciable retention of a protein and it is recommended in the majority of the cases.This resin works well with relatively easy to separate His-tagged proteins.

His-Column High Density Nickel is a Pre-Packed ready to use product for gravity flow purifications. It includes 8 Pre-packed columns filled with 1ml of High Density Nickel. This resin provides a higher amount of available groups and usually allows for higher binding capacity.

Purchase options

Vol

8 x 1 ml $174.88

$174.88

Ask for a bulk quote

Select a purchase option to buy

Related products

Affinity Chromatography

Low Density Zinc

6BCL-QLZn

Zinc chelates seem to recognize two exposed histidine tags in vicinal position – this resin is recommended working with proteins that are difficult to separate. Low Density Zinc has a lower amount of available groups that usually improve the selectivity/specificity. The resin is adequate to work in batch or column purifications (Low Flow). ABT offers...

Affinity Chromatography

Nickel NTA Agarose Resin

6BCL-NTANi

This resin consists of crosslinked agarose derivatized with Nitrilotriacetic acid (NTA) and provides good properties working in native or denaturing conditions. This resin can recover His-tagged proteins from a variety of expression systems such as baculovirus, yeast, mammalian and bacterial cells. ABT offers 25ml, 100ml & 500ml. Bulk quantities...

Affinity Chromatography

Protein L Agarose Resin

4BCLPL

Protein L is an immunoglobulin-binding protein that was isolated from the bacteria Peptostreptococcus magnus and provides a convenient way to separate immunoglobulins from a variety of sources. rProtein L contains four immunoglobulin binding domains of the native protein and may be used for the purification of IgG, IgM, IgA and IgD containing kappa light...

Affinity Chromatography

High Density Cobalt

6BCL-QHCo

Cobalt chelates recognize two exposed vicinal histidine tags - this resin is used in many purifications because this cation provides very good selectivity. HIGH Density COBALT provides a high amount of available groups allowing for higher binding capacity. The resin is adequate to work in batch or column purifications (Low Flow). ABT offers 25ml and...

Affinity Chromatography

Nickel Agarose Extrachel™

6RREXCH

Nickel Agarose Extrachel™ is a high capacity resin manufactured with a polychelator ligand. The product is developed to work in the presence of EDTA and DTT without any loss of performance. Its specificity and stability allows a one-step purification eliminating the need of pretratment of samples that cause nickel stripping.

Affinity Chromatography

Nickel Rapid Run™

6NiRR

Nickel chelate resins recognize two exposed histidine tags for an appreciable retention of a protein and it is recommended in the majority of the cases- this resin works well with relatively easy to separate His-tagged proteins. NICKEL RAPID RUN™ provides the advantages of the Nickel cation with the high flow rates of the Rapid Run™ beads. It is specially...

Affinity Chromatography

Protein G Agarose Resin 4 Rapid Run™

4RRPG

PROTEIN G Agarose Resin 4 RAPID RUN™ consists of Protein G covalently bound to highly crosslinked agarose beads. It provides a very stable bond that can greatly minimize leakage of the Protein G allowing for reuse of the affinity resin in several purification steps. The resin works well in High Flow conditions. ABT offers 1ml, 5ml & 25ml. Bulk...

Affinity Chromatography

Low Density Copper

6BCL-QLCu

Copper chelates recognize one single exposed histidine tag – this resin is recommended working with proteins that are difficult to separate. LOW Density COPPER has a lower amount of available groups that usually improve the selectivity/specificity. The resin is adequate to work in batch or column purifications (Low Flow). ABT offers 25ml and 100ml. For...

Affinity Chromatography

Low Density Metal Free

6BCL-QL

This resin provides a low amount of activation for metal attachment allowing for higher selectivity/specificity in the binding of the tagged-protein. The resin is adequate to work in batch or column purifications (Low Flow). It is supplied without any metal and it is necessary to charge the resin using a selected divalent cation following instructions....

Affinity Chromatography

HisXL Column High Density Cobalt

6BCL-QHCo-C5

Cobalt chelates recognize two exposed vicinal histidine tags - this resin is used in many purifications because this cation provides very good selectivity. His-XL COLUMN HIGH Density COBALT is a Pre-Packed ready to use product for gravity flow purifications. It includes 5 Pre-Packed columns filled with 5ml of HIGH Density COBALT. This resin provides a...

Affinity Chromatography

High Density Zinc

6BCL-QHZn

Zinc chelates seems to recognize two exposed histidine in vicinal position and it is recommended working with proteins that are difficult to separate. HIGH Density Zinc resin provides a high amount of available groups allowing for higher binding capacity. The resin is adequate to work in batch or column purifications (Low Flow). ABT offers 25ml and...

Affinity Chromatography

Biotin Agarose Resin

4BCL-BI

Biotin Agarose Resin is used for purification or removal of avidin or streptavidin samples. Biotin is immobilized through a spacer arm by means of covalent binding that minimize leakage. The binding is very strong, making it suitable for non-reversible binding applications (e.g. removal of avidin components from a sample).

Looking for a custom resin?

The customization of agarose resins is our flagship services, and we develop various custom resins that meet the unique challenges of our clients.

Our custom range also includes agarose beads of various sizes with appropriate separation properties.

Cookie name	Provider	Purpose	Expiry
cookiesplus	https://abtbeads.com	Stores your cookie preferences.	1 year
PrestaShop-#	https://abtbeads.com	This cookie helps keep user sessions open while they are visiting a website, and help them make orders and many more operations such as: cookie add date, selected language, used currency, last product category visited, last seen products, client identification, name, first name, encrypted password, email linked to the account, shopping cart identification.	480 hours

Cookie name	Provider	Purpose	Expiry
collect	Google	It is used to send data to Google Analytics about the visitor's device and its behavior. Track the visitor across devices and marketing channels.	Session
r/collect	Google	It is used to send data to Google Analytics about the visitor's device and its behavior. Track the visitor across devices and marketing channels.	Session
_ga	Google	Registers a unique ID that is used to generate statistical data on how the visitor uses the website.	2 years
_gat	Google	Used by Google Analytics to throttle request rate	1 day
_ga_#	Google	Used by Google Analytics to collect data on the number of times a user has visited the website as well as dates for the first and most recent visit.	2 years
_gd#	Google	This is a Google Analytics Session cookie used to generate statistical data on how you use the website which is removed when you quit your browser.	Session
_gid	Google	Registers a unique ID that is used to generate statistical data on how the visitor uses the website.	1 day

His-Column High Density Nickel

Product info

Overview

Resume

Description

Related documents

Purchase options

Related products

Looking for a custom resin?