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Affinity Coupling
High Density Glyoxal 6BCL
High Density Glyoxal 6BCL allows a covalent binding of agarose to lysine amino groups. This product is adequate to work in batch or column purifications (Low Pressure). The resin has a high amount of aldehydes groups (40-60 µmoles Glyoxal /ml gel) providing a very good option to conjugate affinity ligands improving the stability of the ligand. The backbone of the resin is 6% agarose beads and the pore size is most commonly used to work with enzymes & proteins.
REFERENCES:
- Guisan, Jose M. et al. “One-Point Covalent Immobilization of Enzymes on Glyoxyl Agarose with Minimal Physico-Chemical Modification: Immobilized ‘Native Enzymes.’” Methods in Molecular Biology. Vol. 2100. Humana Press Inc., 2020. 83–92. Methods in Molecular Biology.