6% BCL Agarose Bead Standard (50-150µm)

6% BCL Agarose Bead Standard is a crosslinked agarose resin with a bead size of 50-150 µm. It is a gel filtration resin commonly used for coupling affinity ligands. The matrix is not pre-activated so the user needs to generate groups for coupling procedures.

REFERENCES:

  • Benítez-Mateos, Ana I. et al. “Expanding One-Pot Cell-Free Protein Synthesis and Immobilization for On-Demand Manufacturing of Biomaterials.” ACS Synthetic Biology 7.3 (2018): 875–884. ACS Synthetic Biology. 
  • Yilmaz, Gulden, Esther E. Biswas-Fiss, and Subhasis B. Biswas. “Genetic Variations in the DNA Replication Origins of Human Papillomavirus Family Correlate with Their Oncogenic Potential.” Biochimica et Biophysica Acta - General Subjects 1862.4 (2018): 979–990. Biochimica et Biophysica Acta - General Subjects. 
  • Ruzic, Lucija, Juan M. Bolivar, and Bernd Nidetzky. “Glycosynthase Reaction Meets the Flow: Continuous Synthesis of Lacto-N-Triose II by Engineered β-Hexosaminidase Immobilized on Solid Support.” Biotechnology and Bioengineering 117.5 (2020): 1597–1602. Biotechnology and Bioengineering.
  • Claudia Ubilla et al. "Synthesis of Lactulose in Continuous Stirred Tank Reactor With β-Galactosidase of Apergillus oryzae Immobilized in Monofunctional Glyoxyl Agarose Support" Biotechnology and Bioingenieering. Volume 8, 669 (2020). 

  • Product Name 6% BCL Agarose Bead Standard (50-150µm)
  • Cat. No. A-1061S-X
  • Bead Geometry & Size Spherical ~50-150 µm
  • Crosslinked Yes
  • Agarose % 6 %
  • Bead Mean Diameter d50v ~90 µm
  • Antimicrobial Agent 20% Ethanol
  • Storage Temperature 2-30˚C
  • Exclusion Limit Da >4x10^6
  • pH Stability Working Range 3-13
  • ph stability cleaning in place (cip) 2-14
  • Fractionation (mw) Globular Proteins Da 1x10^4 - 4x10^6
  • Linear Recommended Flow Rate <30 cm/h
  • Chemical Stability Stable in all solutions commonly used in gel filtration, 2 M NaOH, 8 M urea, 6 M guanidine hydrochloride, 30% isopropanol, 70% Ethanol and commonly used detergents
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