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ABT has been awarded the "Innovative SME" label by the Spanish Ministry of Science, Innovation and Universities
This seal confirms the company's commitment to research, custom development and innovation in the field of bioprocessing.
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Next stop: The industry's top trade shows
Agarose Bead Technologies (ABT) is excited to announce our participation in several important trade fairs this October.
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Agarose Bead Technologies expands facility to meet rising global demand for novel therapies market
Agarose Bead Technologies has enhanced its Burgos facility by boosting its agarose resin production to 100,000 liters annually to position itself as a powerhouse in the novel therapies market.
Read moreSee you at Bioprocess International Conference and Exhibition 2024
ABT will be exhibiting at one of the most prestigious events in the bioprocessing industry, the Bioprocess International Conference. This prestigious event will be held...
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"Agarose-based Ion Exchange Chromatography (IEX) resins, such as those offered by ABT, are ideal for oligonucleotide purification workflows"
Oligonucleotides (ONs) have become important molecules in therapy and diagnostics.
A recent comparative study published in Journal of Chromatography A evaluates two prevalent techniques for preparative oligonucleotide purification: Ion Exchange Chromatography (IEX) and Ion-Pair Reversed-Phase Liquid Chromatography (IP-RPLC).
Using a crude 20-mer DNA oligonucleotide, the study investigated key parameters such as gradient slope and column load under realistic laboratory conditions.
The results highlight that agarose-based IEX resins offer superior productivity and solvent efficiency compared to silica-based IP-RPLC media (Figure 1).
Despite longer cycle times, IEX achieved significantly higher loadability—translating into 2 to 7 times greater productivity depending on the target purity (95–99%)—while consuming only one-third to one-tenth the amount of solvent.
The anti-Langmuirian elution profile observed in IEX further contributed to better separation efficiency and reduced loss of the full-length product at high purities.
Figure 1. A comparison of agarose-based Ion Exchange Chromatography (IEX) and silica-based Ion-Pair Reversed-Phase Liquid Chromatography (IP-RPLC) for oligonucleotide purification, highlighting loading capacity, productivity, and solvent use
It is concluded that IEX is the preferred technique for large-scale oligonucleotide purification, especially when high throughput and environmental considerations are prioritized. Additionally, IEX operates at ambient temperature and does not require toxic organic solvents or ion-pairing reagents.
These findings support the use of agarose-based IEX resins, such as those offered by ABT (Agarose Bead Technologies). ABT’s Q Rapid Run™ (High Sub) Agarose Beads are strong anion exchange resins that are ideal for oligonucleotide purification workflows.
The beads offer high dynamic binding capacities, gentle processing conditions, and scalable performance from the laboratory to production scale.
- Martin Enmark, Cecilia Unoson, Marek Leśko, Olof Stålberg, Kathrin Stavenhagen, Manasses Jora, Tomas Leek, Linda Thunberg, Kayla Borland, Jörgen Samuelsson, Torgny Fornstedt. A comparative study of ion exchange vs. ion pair chromatography for preparative separation of oligonucleotides. Journal of Chromatography A, Volume 1746, 2025, 465790